ECTSProtein Biochemistry and Proteomics

Protein Biochemistry and Proteomics (CEAC515) Course Detail

Course Name Course Code Season Lecture Hours Application Hours Lab Hours Credit ECTS
Protein Biochemistry and Proteomics CEAC515 Elective Courses 3 0 0 3 5
Pre-requisite Course(s)
Course Language English
Course Type Technical Elective Courses
Course Level Ph.D.
Mode of Delivery
Learning and Teaching Strategies Lecture, Demonstration, Question and Answer.
Course Coordinator
Course Lecturer(s)
  • Assoc. Prof. Dr. S.Belgin İşgör
Course Assistants
Course Objectives To identify the integration of cellular components and biochemical reactions and to explore proteomics tools to investigate protein levels,localization and function.
Course Learning Outcomes The students who succeeded in this course;
  • Brief explanation of protein structure and function
  • To give information about protein determination methods and their applications
  • To introduce the methods for isolation, purifiation and calculation of the protein concentrations
  • To give information about blotting techniques and staining of proteins
  • To introduce the principles of proteomics.
  • To introduce the mass spectrometer for protein analysis
  • 2D electrophoresis and their applications
  • Protein and peptide sequencing
Course Content Protein concentration calculation, electrephoresis gels; SDS gels, native gels, staining gels, precipitation and concentration of proteins, blotting and protein staining on blots, immunostaining, proteomics principles, 2D electrophoresis, mass spectroscopy of peptides and proteins, mass spectrometers.

Weekly Subjects and Releated Preparation Studies

Week Subjects Preparation
1 Amino acids and protein structure
2 Protein purification methods
3 Protein analysis methods : Bradford method, Biuret Method
4 Protein analysis methods; Lowry method, BCA method
5 Electrophoresis, SDS gels, staining gels.
7 Blotting techniques and protein stainings on blotting
8 Immunostaining methods
9 Autoradiography of gels and blots
10 Ligand binding and radioactive ligand marking
12 Proteomics principles and their applications
13 Mass spectrophotometers for peptides and proteins
14 The working principles of mass spectrophotometer and it is use
15 Microsequencing; Edman Degredation, carboxyterminal sequencing, ladder sequencing of peptides


Evaluation System

Requirements Number Percentage of Grade
Attendance/Participation - -
Laboratory - -
Application - -
Field Work - -
Special Course Internship - -
Quizzes/Studio Critics - -
Homework Assignments 2 15
Presentation - -
Project - -
Report - -
Seminar - -
Midterms Exams/Midterms Jury 2 50
Final Exam/Final Jury 1 35
Toplam 5 100
Percentage of Semester Work
Percentage of Final Work 100
Total 100

Course Category

Core Courses
Major Area Courses X
Supportive Courses
Media and Managment Skills Courses
Transferable Skill Courses

The Relation Between Course Learning Competencies and Program Qualifications

# Program Qualifications / Competencies Level of Contribution
1 2 3 4 5
1 An ability to access, analyze and evaluate the knowledge in chemistry and comprehend the literature. X
2 An ability to define an advanced scientific problem in the required field and finding an alternative for the solution. X
3 An ability to design and conduct scientific and technological experiments in the lab- and pilot-scale, and to analyze and interpret their results. X
4 An ability to perform independent research. X
5 An ability to give a presentation at national and international scientific conferences and to publish scientific publications in international journals. X
6 An ability to achieve the necessary knowledge to follow current developments in science and technology, and do scientific research or developing projects in the field of chemistry. X
7 An ability to work in a multi-disciplinary environment and to work as a part of a team. X
8 An understanding of the professional and occupational responsibilities. Awareness and responsibility about professional, legal, ethical and social issues in the required field. X

ECTS/Workload Table

Activities Number Duration (Hours) Total Workload
Course Hours (Including Exam Week: 16 x Total Hours)
Special Course Internship
Field Work
Study Hours Out of Class 3 10 30
Presentation/Seminar Prepration
Homework Assignments 2 4 8
Quizzes/Studio Critics
Prepration of Midterm Exams/Midterm Jury 2 10 20
Prepration of Final Exams/Final Jury 1 20 20
Total Workload 78