Cell Culture Techniques (CEAC514) Course Detail

Course Name Course Code Season Lecture Hours Application Hours Lab Hours Credit ECTS
Cell Culture Techniques CEAC514 3 0 0 3 5
Pre-requisite Course(s)
Course Language English
Course Type N/A
Course Level Natural & Applied Sciences Master's Degree
Mode of Delivery Face To Face
Learning and Teaching Strategies Lecture, Demonstration, Question and Answer.
Course Coordinator
Course Lecturer(s)
  • Assoc. Prof. Dr. S. Belgin İşgör
Course Assistants
Course Objectives To provide knowledge about animal cell culture which has become an important and essential laboratory technique for the study of biochemical, physiological and pharmacological processes.
Course Learning Outcomes The students who succeeded in this course;
  • Learns the importance of technologic develpments in science
  • Learns the biological role of cellular components and their differences in tissues.
  • to learn aseptic techniques and the use of cell culture laboratory instruments
  • To learn differences between organ, tissue and cell cultures
  • to learn how to select cell lines and their sepplementary media
  • To learn the basics for preparing suspension culture of cell lines from frozen stocks
  • To learn the basics for preparing adherent culture of cell lines from frozen stocks
Course Content Basic cell culture techniques for bioscience research and commercial applications. introduction to cell cultivation methods, introduction to proper use of biological safety cabinet,sterile technique, cell enumaration and media preparartion,primary cultures,cultivation of cell lines, detection of contamination,cryopreservation,transfection,and an

Weekly Subjects and Releated Preparation Studies

Week Subjects Preparation
1 Historical Background in the advancement of TCC Technology. Basic information on cell: cell membrane and its function, organelles, general types of tissues of human origin
2 Tissue Cell Culture Technology: Comparing organ, tissue and cell cultures
3 Basics for primary and secondary cell cultures
4 TCC techniques: Basic aseptic rules
5 TCC techniques: Basic instrumantation
6 TCC techniques: Basic labwares
7 TCC techniques: 4.Cell line selection and supplementary media
8 TCC techniques: 5.Seeding of cells(inoculation), passaging, harvesting of cells and cryopreservation
9 Midterm
10 TCC techniques: 6. Calculation of Growth rate in terms of growth curve and duplication time
11 TCC techniques: 7. Cell viability and cell density calculations
12 TCC techniques: 8. Basics for preparing suspension culture of cell lines from frozen stocks
13 Midterm
14 TCC techniques: 9. Basics for preparing adherent culture of cell lines from frozen stocks
15 Biomedical and Industrial approach to TCC Technology
16 Final


Evaluation System

Requirements Number Percentage of Grade
Attendance/Participation - -
Laboratory - -
Application - -
Field Work - -
Special Course Internship - -
Quizzes/Studio Critics - -
Homework Assignments - -
Presentation - -
Project - -
Report - -
Seminar - -
Midterms Exams/Midterms Jury 2 25
Final Exam/Final Jury 1 35
Toplam 3 60
Percentage of Semester Work
Percentage of Final Work 100
Total 100

Course Category

Core Courses X
Major Area Courses
Supportive Courses
Media and Managment Skills Courses
Transferable Skill Courses

The Relation Between Course Learning Competencies and Program Qualifications

# Program Qualifications / Competencies Level of Contribution
1 2 3 4 5
1 An ability to access, analyze and evaluate the knowledge needed for the solution of advanced chemical engineering and applied chemistry problems.
2 An ability to self-renewal by following scientific and technological developments within the philosophy of lifelong learning.
3 An understanding of social, environmental, and the global impacts of the practices and innovations brought by chemistry and chemical engineering.
4 An ability to perform original research and development activities and to convert the achieved results to publications, patents and technology.
5 An ability to apply advanced mathematics, science and engineering knowledge to advanced engineering problems.
6 An ability to design and conduct scientific and technological experiments in lab- and pilot-scale, and to analyze and interpret their results.
7 Skills in design of a system, part of a system or a process with desired properties and to implement industry.
8 Ability to perform independent research.
9 Ability to work in a multi-disciplinary environment and to work as a part of a team.
10 An understanding of the professional and occupational responsibilities.

ECTS/Workload Table

Activities Number Duration (Hours) Total Workload
Course Hours (Including Exam Week: 16 x Total Hours) 14 3 42
Special Course Internship
Field Work
Study Hours Out of Class 1 12 12
Presentation/Seminar Prepration
Homework Assignments
Quizzes/Studio Critics
Prepration of Midterm Exams/Midterm Jury 2 12 24
Prepration of Final Exams/Final Jury 1 36 36
Total Workload 114